Question: A cross is made between the streptomycin - resistant ( Str ) F - strain marked with the characters Gal T ARLac T ] R
A cross is made between the streptomycinresistant Str F strain marked with the characters Gal T ARLac TR Mal Xyl Li and the prototrophic Hfr strain having the opposite characters. After minutes d contact, samples are transferred to plates with minimal medium plus streptomycin. All markers can be transferred within this time. Key to characters: T and L are amino acids; Gal, Lac, Mal, Xyl are sugars; Az azide; is phage; Str streptomycin. The original mixture is in the ratio of x Hfr to x FThe frequencies percentages of the fr characters recovered in the TL SR recombinants are as follows: T Mal Gal AzS, Xyl Lac Note: The order of T and L and Mal and Xyl cannot be determined from these data. pta What is the counterselective agent that prevents the Hfr individuals from obscuring the detection of recombinants?b In what order are these genes probably being transmitted by the HfrTL must come in first, and they must be closely linked.c What is the meaning of Mal and Xyl Where do you think these markers are located on the chromosome relative to StrR
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