Question: A cross is made between the streptomycin - resistant ( Str ) F - strain marked with the characters Gal T ARLac T ] R

A cross is made between the streptomycin-resistant (Str) F- strain marked with the characters Gal T ARLac T]R Mal Xyl Li and the prototrophic Hfr strain having the opposite characters. After 60 minutes d contact, samples are transferred to plates with minimal medium plus streptomycin. All markers can be transferred within this time. Key to characters: T and L are amino acids; Gal, Lac, Mal, Xyl are sugars; Az= azide; 71 is phage; Str = streptomycin. The original mixture is in the ratio of 2 x 10' Hfr to 4 x 108 F-.The frequencies (percentages) of the fr characters recovered in the T+L+ SR recombinants are as follows: 72% T/,0% Mal+,27% Gal+,91% AzS, 0% Xyl,48% Lac+. Note: The order of (T and L) and (Mal and Xyl) cannot be determined from these data. (6 pt)67.(a) What is the counterselective agent that prevents the Hfr individuals from obscuring the detection of recombinants?68.(b) In what order are these genes probably being transmitted by the Hfr?(T+L+ must come in first, and they must be closely linked.)69.(c) What is the meaning of 0% Mal+ and 0% Xyl+? Where do you think these markers are located on the chromosome relative to StrR?

Step by Step Solution

There are 3 Steps involved in it

1 Expert Approved Answer
Step: 1 Unlock blur-text-image
Question Has Been Solved by an Expert!

Get step-by-step solutions from verified subject matter experts

Step: 2 Unlock
Step: 3 Unlock

Students Have Also Explored These Related Biology Questions!