Question: A DNA fragment has the sequence 5 'ATTACG 3 ' . Assuming standard biochemical conditions ( RT = 0 . 6 Kcal / mol )

A DNA fragment has the sequence 5'ATTACG3'. Assuming standard biochemical conditions (RT =0.6 Kcal/mol) and that Go' for a Watson-Crick hydrogen bond is approximately -0.9 kcal/mol, calculate a best estimate for the dissociation constant for the helix association reaction:
DNA sequence fragment + complementary DNA sequence fragment -----> DNA helix

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