Question: a) Draw an in-gel fluorescence experiment showing what competitive Activity-Based Protein Profiling (ABPP) would look like for a selective (A), partially selective (B) and
a) Draw an in-gel fluorescence experiment showing what competitive Activity-Based Protein Profiling (ABPP) would look like for a selective (A), partially selective (B) and non-selective inhibitor (C) for the cysteine protease Cathepsin B. b) For the same enzyme draw a fluorescence gel for an ABPP experiment showing in vivo inhibition of the target enzyme in a mouse by two different inhibitors (D, and E) showing good and poor bioavailability. c) Draw a fluorescence gel for an ABPP experiment showing tissue-specific enzyme labeling of Cathepsin B. Q2. 3
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