7. Protein Blue dextran A series of proteins with known molecular mass and an enzyme of...
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7. Protein Blue dextran A series of proteins with known molecular mass and an enzyme of unknown molecular mass are separated by chromatography on a Sephadex G-200 column. The elution volume (Ve) for each protein is indicated in the table below. Estimate the molecular mass of the unknown protein. lysozyme Chymotrypsinogen ovalbumin Serum albumin aldolase urease ferritin ovomucoid Unknown 8. Indicats M₁ 1000 kDa 14 kDa 25 kDa 45 kDa 65 kDa 150 kDa 500 kDa 700 kDa 28 kDa ? Ve (ml) 85.00 200.00 190.00 170.00 150.00 125 90.00 92.00 160.00 130.00 7. Protein Blue dextran A series of proteins with known molecular mass and an enzyme of unknown molecular mass are separated by chromatography on a Sephadex G-200 column. The elution volume (Ve) for each protein is indicated in the table below. Estimate the molecular mass of the unknown protein. lysozyme Chymotrypsinogen ovalbumin Serum albumin aldolase urease ferritin ovomucoid Unknown 8. Indicats M₁ 1000 kDa 14 kDa 25 kDa 45 kDa 65 kDa 150 kDa 500 kDa 700 kDa 28 kDa ? Ve (ml) 85.00 200.00 190.00 170.00 150.00 125 90.00 92.00 160.00 130.00
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Blue dextran used to get the idea of void volume Vo The princi... View the full answer
Related Book For
Chemistry The Central Science
ISBN: 978-0321696724
12th edition
Authors: Theodore Brown, Eugene LeMay, Bruce Bursten, Catherine Murphy, Patrick Woodward
Posted Date:
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