Question: a) Yeast is to be produced in continuous culture using glucose as the limiting substrate. The chemostat has a working volume of 100m3, cell growth

a) Yeast is to be produced in continuous culture using glucose

a) Yeast is to be produced in continuous culture using glucose as the limiting substrate. The chemostat has a working volume of 100m3, cell growth can be adequately described by the Monod equation and the organism has the following specific characteristics: Monod's Constant (or Substrate affinity constant): Ks=0.1kgm3 Maximum specific growth rate: max0.4h1 Biomass yield on glucose: Yx/s=0.4 i) Starting from mass balances, mathematically show how the steady-state biomass [50] concentration (CX) vary in a well-mixed chemostat with single limited nutrient. Clearly state all assumptions made. ii) Calculate the steady-state substrate and biomass concentrations when the input [20] rate of feed is 20m3h1 and the glucose concentration is 40kgm3. b) Graphically show how the concentration of biomass and substrate vary with the dilution [30] rate in a CSRR. Please mark all the important regions and the values in your graph

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