Question: Spotting refers to: a). An unwanted creation of Dalmatians during a chemical reaction; b). The application of a compound or mixture to a TLC plate

"Spotting" refers to: 

a). An unwanted creation of Dalmatians during a chemical reaction;

b). The application of a compound or mixture to a TLC plate using a micro capillary tube; 

c). The application of very small Dalmatians to a TLC plate using a microcapillary tube; 

d). An unwanted distribution of compounds on a TLC plate based on solubilities that are incompatible with the plate.

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