Question: A16 Compound A is the substrate for two enzymes, E, and E,, involved in the synthe- sis of an amino acid and an antibiotic, respectively.

 A16 Compound A is the substrate for two enzymes, E, and

A16 Compound A is the substrate for two enzymes, E, and E,, involved in the synthe- sis of an amino acid and an antibiotic, respectively. Their reaction rates, r, and r'a, at an arbitrary concentration of E, and E, were measured at different concen- trations of A. However, the data were not labeled properly (shown in Table P.4.1), and the student who did the measurement needs help to sort out which dataset is for E. Determine the Km and r max for both enzymes, with respect to the concen- tration of A. Which set of data is more likely to be for E, and which for Ey, and why? 0.2 8 9 12 15 Table P.4.1 Reaction kinetics of a common substrate A for two enzymes. Concentration of 0.6 1.2 2 3 4 5 6 A (MM) Reaction rate (r) 3.33 4.29 4.62 4.76 4.84 4.88 4.9 4.92 (mmol/L*min) Reaction rate (r) 0.09 0.23 0.38 0.5 0.6 0.67 0.71 0.75 (mmol/L*min) 4.94 4.95 4.96 4.97 0.8 0.82 0.86 0.80

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