An experimenter performs two sets of enzyme kinetics experiments. One set of experiments, when plotted as a double-reciprocal plot, yielded a y-intercept of 0.043 M-1min and an x-intercept of -20.0 mM-1. A second set of experiments, when similarly plotted, yielded a y-intercept of 0.129 M-1min and an x-intercept of -60.0 mM-1. The second set of experiments contained 89 nM of an uncompetitive inhibitor. Given these data, what is the Ki' (in nM to the nearest tenths) for this inhibitor? Hint: If you do not recall what the x-intercept represents, you can write the double-reciprocal equation and set 1/v equal to zero. Also, for this problem, you can assume that the enzyme exhibits Michaelis-Menten kinetics and that the estimates for Vmax and Km that are obtained from a Lineweaver-Burke (i.e., double-reciprocal) plot are satisfactory. (Recall that I advocated in class for the use of nonlinear fitting algorithms.)