answer all questions

1. Write a short description of absorption spectroscopy, include what the underlying theory is, the purpose of a monochromater, and how absorbance is determined in Equation (1) of the lab handout. 2. Explain what Equation (2) for the Beer-Lambert Law represents, define its terms, and describe how it is used to determine the concentration of an unknown sample. 3. a. Explain how direct monitoring by UV absorbance measurements at 280nm is used to determine protein concentrations in samples. What are the advantages and disadvantages of this method? b. How is the Bradford reagent used to determine protein concentrations in solutions? What are the advantages and disadvantages of this method