Question: answer Biophysical Techniques in Biochemistry - Exam IV Name: Question 2 Answer one out of two application questions. Application 1: LPS Overlay Overlay 20 -15

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Biophysical Techniques in Biochemistry - Exam IV Name: Question 2 Answer one out of two application questions. Application 1: LPS Overlay Overlay 20 -15 - Frequency Shit!" " 9 mM EDTA 9.0 mM EDTA MSI-78+9mM EDTA MEDIA-30% MSL TA Note 1: The above figure shows the asymmetric 2.5 mM EDTA outer membrane of bacteria, which contains MSI-78 lipopolysaccharide (LPS) with divalent cations 2.5 mMEDIA Mg2* and Ca2* interspersed. Control Note 2: EDTA treatment is known to chelate (bind Control to) any available divalent cations. No Treatment -60 40 0 20 -100 -80 60 40 -20 0 20 Frequency (KHz) Frequency (KHz) The above 2H NMR spectra depict the effect of antimicrobial peptide (AMP) MSI-78 and EDTA treatments on live bacteria. a) What is the antimicrobial peptide (MSI-78) doing to the sample? Explain how you came to your conclusion. (1.5 pt) b) What is EDTA doing to the sample? Explain how you came to your conclusion. Also, explain any limitations in your conclusion based on the limited information provided to you. (3 pt) c) Based on your answers from 2a and 2b, do the divalent cations stabilize or destabilize the bacterial outer membrane? (0.5 pt)

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