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der 152 Microbiology - Laboratory Manual 326 Case Study Exercise 36 Isolation and Identification of Staphylococc PRE-LAB QUESTIONS Remember, Understand, Apply 5. Which type of media is used to determine the species of Staphylococcus in an isolate? 1. What percentage of the population carries Staphylococcus a. selective media aureus in their nose? ( differential media a. 2% 6. Define the following terms using the laboratory exercise b. 15% or glossary: c. 33% Fomite An object of material likely (.) 50% too c carryinfection ,such as clothes, 2. Carriers of Staphylococcus aureus have no symptoms and Utensils, and furniture. don't know they are carriers unless they have a culture performed. Coagulase - An enzyme produced by @ true Save bacteria that enables the b. false conversion of fibrinogen or fiber 3. Which of the following traits are characteristics of Aiding in clot formation. Staphylococcus aureus (choose all that apply)? 7. Explain the selective and differential properties of mannito a. resistant to drying salt agar. b. resistant to extremes in pH Mannitol spit Ager is selective bus c. resistant to high temperatures contains a high concentration uf belt, wh inhibits the grouth of most backria exing d. resistant to high salt (7.5%) simplylocali differtol because it condon e. resistant to high sugar concentrations 8. Using Table 36.1 as a guide, fill in the middle column in th accompanying table with a single characteristic that wou 4. MRSA infections have only been associated with health allow you to distinguish between the two species preser care settings. in the first column. Fill in the right-hand column in a simil a. true fashion, indicating what medium or test could be used b, false A&M differentiate the organisms in each pair. Medium or test used Organisms Characteristic used to differentiate organisms S. aureus vs. S. epidermidis S. epidermidis vs. S. saprophyticus S. aureus vs. S. saprophyticus 3. Moisten a second swab by immersing it partially PERIOD ONE tube of broth labeled "fomite." Use this swab to surface of a fomite, and place the swab into the t MATERIALS 4. Using the tongue depressor to hold down the to final swab to sample the back of your lab partne Each student should obtain: Avoid touching the tongue or cheeks with the s Three tubes of m Staphylococcus broth swab into the throat tube. Dispose of the tong a hard-sided biohazard container. the at 37 C for 24 h