Answer each statement with a true or false

1) Proteins become positively charged interaction with SDS in the loading buffer

2) SDS-PAGE separates proteins based on their isoelectric point

3) 1,4-Dithiothreitol (DTT) is a reducing agent

4) SDS-PAGE separates proteins based on their molecular weight (i.e. number of amino acids)

5) When Bradford Reagent reacts with protein the solution becomes a red-brown colour.

6) In SDS-PAGE proteins are separated based on their secondary structure

7) Bradford reagent is a skin irritant

8) Bradford Reagent is toxic by ingestion

9)Ammonium persulfate (APS) is a catalyst that accelerates gel polymerization

10) SDS is harmful by ingestion

11) The resolving and stacking gels contain similar % acrylamide content

12) SDS-PAGE separates proteins based on their net charge

13) Proteins refold when they enter the resolving gel

14) -mercaptoethanol is a reducing agent

15) In SDS PAGE proteins migrate toward the cathode

16) Higher acrylamide content in SDS-PAGE gels aids the separation of smaller proteins