Question: Answer it plz, tnxx 15. A new method is proposed to simultaneously detect and separate four food additives by reversed phase liquid chromatography. The data

Answer it plz, tnxx

Answer it plz, tnxx 15. A new method is proposed to simultaneously

15. A new method is proposed to simultaneously detect and separate four food additives by reversed phase liquid chromatography. The data were obtained using the liquid chromatographic column with a flow rate of 0.5 mL per min and with the following parameters: Pore size 100 A Particle size 3.5 um Internal diameter 4.6 mm Length 150 mm The food additives found in the sample resulted in the following data: Caffeine Aspartame Benzoic acid Sorbic acid Retention time (min) Width at peak base 1.05 5.560 2.65 9.787 4.00 12.053 2.58 18.511 3.96 i Calculate the number of plates from each peak ii Calculate the plate height for the column. ii Retention factor for each peak. iv Determine the resolution factor for analytes caffeine and aspartame. v Determine the separation factor for analytes caffeine and aspartame. vi What will be the length of the column to acquire a resolution of 2.0 for caffeine and aspartame? vii If the resolution will be 2.0, how much time is required to separate the caffeine and aspartame? viii What would be the optimum length for benzoic acid and sorbic acid to have a resolution of 2.0

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