Question: answer quedtions 1,3,&5 please answer each question they are on seperate photos for better quality 1) The following data were obtained for a new protein


1) The following data were obtained for a new protein structure. What is the likely secondary structure motif for this part of the protein? Refer to Ramachandran Diagram () I del ber 11 61 72 11 4 > 23 31 31 be 1 150 154 10 91 1014 3) What is the pH of a O 3M Lysine solution whose e-NH, is 25% dissociated? Lysine's pka of the a-COOH is 2.16. a-NH, is 9.06 and that of the side-chain is 1054. Show your work (15 pt) 5) Determine the subunit composition of a protein from the following information: Molecular Mass by Gel Filtration: 200 KD Molecular Mass by SDS-PAGE: 100KD Mass by SDS-PAGE with 2-0etsartrethanel 40KD and 60 kD (5 pt) 1) The following data were obtained for a new protein structure. What is the likely secondary structure motif for this part of the protein? Refer to a Ramachandra Diagram (5 pt). I v Residue Number 31 61 72 32 33 34 67 -23 35 21 -51 Break Break Break 2 -30 142 - 142 150 44 -154 121 15 91 136 46 - 110 174 5) Determine the subunit composition of a protein from the following information: Molecular Mass by Gel Filtration: 200 KD Molecular Mass by SDS-PAGE: 100kD Mass by SDS-PAGE with 2-Wersartoethanol 40kD and 60 kD (5 pt) I 3) What is the pH of a 0.3M Lysine solution whose e-NH2+ is 25% dissociated? Lysine's pka of the a-COOH is 2.16, a-NH3* is 9.06 and that of the side-chain is 10.54. Show your work (15 pt)
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