Answer the questions.

PROCESS 1) 80 tomato seeds distributed in a circular shape are placed in petri dishes with moistened filter paper (10 ml. of sterile distilled water). Leave them for 24 hours 2) Subject 40 seeds to magnetic treatment with an induction of 0.1 Tesla at 26C0 approximately in the dark for 8 days. The petri dishes should be placed on the north of one of the magnets 3) After 8 days of induction the seeds must be disinfected before the in vitro germination.

EXPERIMENTAL DEVELOPMENT 1) Treatment I (control) Place 20 seeds without magnetic induction in a semi-solid culture medium 2) Treatment II (control Place 20 seeds without magnetic induction in a liquid culture medium. 3) Treatment III Place 20 seeds with magnetic induction in a semi-solid culture medium 4) Place 20 seeds with magnetic induction in a liquid culture medium. 5) Make daily observations for 30 days 6) Make a graph of % germination vs. time (in days) 7) Calculate the % germination of the seeds for each treatment 8) Carry out the analysis of the data obtained from the observations 9) Do-- an analysis of variance for 95% confidence and a student test using a statistical package Stagraphis version 5.1

DISINFECTION PROTOCOL 1) The seeds are washed with a commercial detergent for 15 minutes (they should make 3 rinses with sterile distilled water) 2) Then they are washed for 3 minutes with 10% sodium hypochlorite for 15 minutes and rinsed 3 times with sterile distilled water. The seeds are placed in the laminar flow cabinet and immersed in 70% ethanol. for 3 minutes and rinsed 3 times with sterile distilled water

QUESTIONS 1) In which of the treatments carried out was the highest percentage of germinated seeds? 2) Do magnetic fields penetrate biological tissues? 3) Do cell membranes have different properties electromagnetic?