Anthropometric measurements including weight (in kg) and height (in cm) were measured in all subjects. Body mass index (BMI) was calculated using the formula: weight (kg)/height 2 (m 2 ). The assessment of TEWL was done in right forearm (extensor area) volunteers. TEWL of forearm was measured using the Tewameter TM300. The temperature in the room was 20C and the relative humidity was 50%. The subjects were acclimatized in the test room 20 minutes before measuring TEWL variables. In summary