as soon as possible please :) equations 7.1 & 2 provided

You measure 2.50mL of a wastewater sample and 3.50mL of Polyseed and place then into a 300mL BOD bottle and fill the remaining volume with BOD dilution water. The Initial D.O. is 9.00mg/L and the Final D.O. is 5.20 mg/L. In your B.O.D. test preparation you recorded the following data for a Seed Control sample consisting of 25.0mL of activated Polyseed: Initial D.O. =8.95mg/L, Final D.O. =4.58mg/L. Use Equation 7.2 to calculate the Seed Control Factor, and then Equation 7.1 to calculate the seed-corrected B.O.D of the wastewater. The D.O. measurements were all at 20C. Final D.O. measurements were made on day 5. All B.O.D. bottles are standard 300mL. The fixed amount of dissolved oxygen (DO) available inside the bottle is consumed by respiration of the aerobic bacteria during the process of decomposing the dissolved organic matter. The DO is measured by using either a standard titration method (called the Winkler titration) or by using an approved DO probe. The BOD of seeded samples is calculated as follows: BODn,mg/L=[(DO1DOn)S.C.F.]Vsample300ml Equation 7.1 DO1=DO of diluted sample immediately after preparation, mg/L DOn=DO of diluted sample after n days of incubation, mg/L Vsmple=v volume of the test sample added to the 300mL-standard size bottle S.C.F. = calculated DO uptake by the seed sample volume added to the bottle (S.C.F. =0 if no seed is used) Equation 7.2 According to the APHA 5210B Method, in the determination of BOD of domestic wastewater, it can be assumed that there is a sufficient and healthy bacterial population to start the "digestion" of organic matter. The method recommends the use of a bacterial seed for samples such as wastewater from an industrial process or chlorinated effluents in which is not certain whether or not sufficient bacteria are present at the start of the test. When a bacterial "seed" (commercial or, for example, cultured in the wastewater plant) is added at the start of the test, a "seed blank" experiment must be prepared in order to determine the DO uptake attributable to the seed added to each bottle, the Seed Correction Factor (S.C.F.), and subtract it from the measured uptakes in each seeded sample bottle as shown in Equation 7.1. In or lab we use the commercial seed Polyseed mM, free of nitrifying microorganisms, and set up Seed Control samples as recommended by manufacturers. The S.C.F. is determined from the BOD resulting from the incubation of this set of Seed Control Samples in conditions identical to all other samples