as standards is used on the same for this have diploid AMY$1$ copy numbers of $3,6,$ and $14,$ respectively. The $\frac{?}{{?}_{\frac{?}{?}}Ct}$ values for the three technical replicates of the known standards are provided.

In Excel $($or Google Sheets$),$ select the data highlighted in yellow in the two columns labeled $"\frac{?}{{?}_{\frac{?}{?}}{C}_t}"$ and "Diploid AMY$1$ Copy#$"$ and use the Scatterplot function $($Insert$->$Chart$->$XY Scatter$)$ to determine the relationship between $\frac{?}{{?}_{\frac{?}{?}}Ct}($independent variable, $x-$axis$)$ and AMY$1$ copy number $($dependent variable, $Y-$axis$).$

Please provide the scatterplot with labeled axes and a Figure caption explaining the plot. Does there appear to be a relationship between $\frac{?}{{?}_{\frac{?}{?}}Ct}$ and diploid AMY$1$ copy number?

Describe the relationship, and explain if this relationship makes sense in light of what you know about how qPCR is sensitive to the initial quantity of template genes present.

$3.$ In Excel $($or Google Sheets$),$ click on your chart and then select "Add Chart Element$>$Trendline$->$Linear". Double click on the trendline and select "Display Equation on chart" and "Display R$-$squared value on chart". The equation represents the least$-$squares best fit linear regression equation for the relationship between $\frac{?}{{?}_{\frac{?}{?}}Ct}$ value and diploid AMY$1$ copy number. The R$-$squared value provides a measure of how well the equation describes the data $($the closer the value is to $1$ or $-1,$ the better the fit$).$

Provide the linear regression equation:

Provide the R$-$square value: