Question: asap please Development of RPLC Method - You have been given an unknown sample mixture, a C18 column and asked to develop a RPLC method.

asap please

Development of RPLC Method - You have been given an unknown sample mixture, a C18 column and asked to develop a RPLC method. -Where do you start? Isocratic or gradient elution? - Using a scouting gradient, the following chromatogram was obtained

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