Assume that you set-up an experiment by seeding Human Mesenchymal Stem Cells (hMSC) on the nanofibers. You have 3 groups and 4 characterization techniques after cell seeding. Experiments should be performed 3 times for the same analysis on the same sample. The concentration of the cells is 3x10⁴cells/cm² and the volume of cell suspension in each nanofiber is 50µL. Each nanofiber is circular with diameter of 12mm. For cell seeding, cell suspension was homogenized carefully mixed 100 μl of cells with 9.9 ml of PBS before counting. Then 50μl of cells with 50 μl of trypan blue were mixed.  75 cells were counted in 5 squares of a hemocytometer

  a-)Please set up an experiment by calculating;

                i-) total sample number

               ii-) total sample volume

               iii-) cell number in each sample 

               iv-) cell number in total volume

b-)What is the concentration of cells in original undiluted culture?

c-)How much cell suspension and medium should be mixed for each sample and total sample?

NOTE: You will obtain 5x10⁴ cells/cm² concentration by mixing cell suspension and medium.