Question: Assume that you should prepare basal medium with DMEM containing 5 % FBS, 100 units/mL penicillin, 100 ug/mL streptomycin, 50 ug/mL gentamicin, and 300 ng/mL

Assume that you should prepare basal medium with DMEM containing 5 % FBS, 100 units/mL penicillin, 100 ug/mL streptomycin, 50 ug/mL gentamicin, and 300 ng/mL amphotericin B for Human Mesenchymal Stem Cell (hMSC) cell line cultivation. Please find required DMEM, FBS, Penicillin/Streptomycin, gentamicin, amphotericin B volume to prepare 250 ml basal medium according to stock solution concentration.

PenStrep Stock: 10,000 U/mL Penicillin and 10,000 ug/mL

Streptomycin Amphotericin B: 250 ug/mL

Gentamicin: 10 mg/uL

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