Assuming the product formed from your enzyme has an absorbance at 405 nm, the following data...
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Assuming the product formed from your enzyme has an absorbance at 405 nm, the following data for constructing a standard curve were obtained *The concentration of Product Stock solution: 50 mM. Tube [Product) Product Stock Buffer Absorbance (mM) solution (ml) (405 nm) (mL) 1 5. 0. 1. 4 0.21 1.5 3.5 0.35 2 3 0.48 4 3.5 1.5 0.83 5. 1.12 6. 3. (1) Calculate the concentration of product [Product) in each tube used to generate the standard curve. Type your answers directly into the answer window. Note: you do NOT need to reproduce the table, just indicate the tube number and concentration. [2 marks) (i) Produce a standard curve using this dataset (you may use excel). Generate a trendline (set y-intercept to zero) and type the equation of the line into the answer window. Note: you do NOT need to shew the standard curve you produced, only the equation of the trendline is required. [2 marks) (i) Use the equation you generated from part (i) to estimate the concentration of product in an unknown sample, given that the Abs at 405 nm of this unknown sample was found to be 0.67. Type your answer into the answer window. (1 marks) Note: Type your answers into the answer window. Please clearly show which answer is for cach part by including (1). (), or (ii) before your answer. Assuming the product formed from your enzyme has an absorbance at 405 nm, the following data for constructing a standard curve were obtained *The concentration of Product Stock solution: 50 mM. Tube [Product) Product Stock Buffer Absorbance (mM) solution (ml) (405 nm) (mL) 1 5. 0. 1. 4 0.21 1.5 3.5 0.35 2 3 0.48 4 3.5 1.5 0.83 5. 1.12 6. 3. (1) Calculate the concentration of product [Product) in each tube used to generate the standard curve. Type your answers directly into the answer window. Note: you do NOT need to reproduce the table, just indicate the tube number and concentration. [2 marks) (i) Produce a standard curve using this dataset (you may use excel). Generate a trendline (set y-intercept to zero) and type the equation of the line into the answer window. Note: you do NOT need to shew the standard curve you produced, only the equation of the trendline is required. [2 marks) (i) Use the equation you generated from part (i) to estimate the concentration of product in an unknown sample, given that the Abs at 405 nm of this unknown sample was found to be 0.67. Type your answer into the answer window. (1 marks) Note: Type your answers into the answer window. Please clearly show which answer is for cach part by including (1). (), or (ii) before your answer.
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Related Book For
Essentials of Statistics for the Behavioral Sciences
ISBN: 978-1285056340
8th Edition
Authors: Frederick J Gravetter, Larry B. Wallnau
Posted Date:
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