Question: B. (25 points) Given the chromatogram below, identify the purity of API (Cas9 RNP in polymersome). Assume the following: 1. Solvent, 2. Free polymer, 3.

B. (25 points) Given the chromatogram below, identify the purity of

B. (25 points) Given the chromatogram below, identify the purity of API (Cas9 RNP in polymersome). Assume the following: 1. Solvent, 2. Free polymer, 3. Cas9 RNP encapsulated in polymersomes, 4. Cas9 RNP aggregates. 3 0.09 2 4 0.06 0.03 0.00 0.0 2.0 1.0 3.0 4.0 5.0 6.0 7.0 8.0 10.0 min 9.0

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