Question: Bandwidth dependence on flow can be described in many ways. In the course, we used the van Deemter equation H = A + B /

Bandwidth dependence on flow can be described in many ways. In the course, we used the van Deemter equation H = A + B/u + C*u.
a) Describe in detail what the constants A, B, and C represent in terms of bandwidth phenomena.
b) Now, if we are to perform three different separations:
(1) SFC for small molecules (with a molecular weight of about 100 g/mol, packing material 10\mu m in diameter);
(2) protein purification (proteins with molecular weights between 60,000 to 200,000 g/mol, packing material 50\mu m in diameter);
(3) the same as in (1) but now with LC instead of SFC.
Discuss and rank from lowest to highest the van Deemter constants A, B, and C for the three different separation systems. Also, discuss why you expect them to be in this particular order.

Step by Step Solution

There are 3 Steps involved in it

1 Expert Approved Answer
Step: 1 Unlock blur-text-image
Question Has Been Solved by an Expert!

Get step-by-step solutions from verified subject matter experts

Step: 2 Unlock
Step: 3 Unlock

Students Have Also Explored These Related Chemistry Questions!