Question: Bandwidth dependence on flow can be described in many ways. In the course, we used the van Deemter equation H = A + B /
Bandwidth dependence on flow can be described in many ways. In the course, we used the van Deemter equation H A Bu Cu
a Describe in detail what the constants A B and C represent in terms of bandwidth phenomena.
b Now, if we are to perform three different separations:
SFC for small molecules with a molecular weight of about gmol packing material mu m in diameter;
protein purification proteins with molecular weights between to gmol packing material mu m in diameter;
the same as in but now with LC instead of SFC
Discuss and rank from lowest to highest the van Deemter constants A B and C for the three different separation systems. Also, discuss why you expect them to be in this particular order.
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