"Based on the data provided, if the A$549$ cells were to be incubated for another $1$ hour at $43$oC$,"$

no changes would be expected in terms of cellular morphology or protein stability

the cells would likely enter S phase and start doubling their DNA

the cells would probably exit the cell cycle and remain in G$0$

the nucleus of the cells would start to form vesicles and the cells would start to lose their normal shape and start forming large buds at the plasma membrane

"the cells would probably become enlarged and some of them might rupture, releasing their cytosolic content to the extracellular environment"

"the cells would probably shrink and decrease in volume and appear deflated, like a ball without air"