Basic classification of polymerases includes how many types?

a) 2

b) 3

c) 4

d) 5

2. Polymerase can be defined as ______________

a) an enzyme used to synthesize a new DNA or RNA strand on the basis of pre-existing strand or at times without a pre-existing strand

b) an enzyme used for removal of nucleotides from the DNA or RNA strand

c) an enzyme which can synthesize only a new DNA strand, not an RNA strand

d) an enzyme which can synthesize either a new DNA or an RNA strand but only when a strand is there

3. Which of the following activity is not possible in the case of DNA polymerase I?

a) 3'-5' exonuclease

b) 5'-3' exonuclease

c) 5'-3' DNA synthesis

d) 3'-5' DNA synthesis

4. The E. coli DNA polymerase enzyme gives different domains with different activities on cleaving with protease subtilisin. Which of the following statements is correct with respect to the fragments generated?

a) The smaller fragment is having C terminal and the larger fragment is having N terminal

b) The smaller fragment is named as Klenow fragment and the intact molecule is called as Kornberg fragment

c) The smaller fragment is having 5'-3' exonuclease activity whereas the larger fragment is having 5'-3' polymerase and 3'-5' exonuclease activity

d) Both the fragments are having 5'-3' polymerase and 3'-5' exonuclease activity

5. Removal of single stranded portions produced due to exonucleolytic activity and due to polymerase activity are termed as _____________

a) polishing and end filling respectively

b) end filling and polishing respectively

c) polishing in both the cases

d) end filling in both the cases

6. Thermostable DNA polymerases are very important in PCR. How are they obtained?

a) They are obtained by heating the bacteria manually over high temperatures

b) They are isolated from extremely stable thermophilic bacteria which are often found growing in oceanic vents

c) They are found everywhere in nature

d) They are obtained by genetically modifying the E. coli bacteria with thermal stability property

7. Which of the following enzyme is said as reverse transcriptase?

a) DNA dependent DNA polymerase

b) RNA dependent RNA polymerase

c) RNA dependent DNA polymerase

d) DNA dependent RNA polymerase

8. Why reverse transcriptase enzymes are having comparatively high error rates than other polymerases?

a) Because they are not having 3'-5' proofreading exonucleolytic activity

b) Because they are not having 5'-3' proofreading exonucleolytic activity

c) Because they are having slow rates of exonucleolytic activity

d) It is difficult to synthesize DNA from RNA

9. Which polymerase can be used in conjunction with appropriate phage promoters in order to have high levels of specific transcription?

a) RNA dependent DNA polymerase

b) DNA dependent RNA polymerase

c) RNA dependent RNA polymerase

d) DNA dependent DNA polymerase