Beer-Lambert law states that the absorbance of light is proportional to the pathlength (I) that light must travel through the sample, the concentration (c) of the sample and the molar attenuation coefficient (, a physical property of each species that affects how much light it will absorb at each wavelength. It can only be determined experimentally.) A = I.C.E Determine what affect the following variations will have on the absorbance of light. Double the concentration of the standard sample is added. Half the concentration of the standard sample is added. Change the cuvette from a 1 cm pathlength to a 2 cm pathlength. Change the cuvette from a 1cm pathlength to a 0.5 cm pathlength. Change the wavelength we are measuring We add move of the same standard solution to the cuvette [Choose ] [Choose ] [Choose ] [Choose ] [Choose ] [Choose ] + + + = <>

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To analyze the effects of the variations on the absorbance of light in the BeerLambert law equation ... View the full answer