Question: Biochem 3390 assignment In practice, ITC data is fitted with a sigmoidal equation to determine a Kd - as shown in class. However this equation
Biochem 3390 assignment In practice, ITC data is fitted with a sigmoidal equation to determine a Kd - as shown in class. However this equation is very difficult to work with and requires a non-trivial fitting algorithm. To simplify things, re-plot your ITC data (Q2.2) using the change in heat per injection to produce a hyperbolic binding curve that utilizes the total concentrations of the RBD (Lt) and ACE2 (Pt) proteins. Plot this new graph and fit the data using the Excel Solver tool (no other program). Submit the fitted graph as a jpg or png file
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