2) Name the three stages of a PCR reaction. For each stage, list its relative temperature (="highest temp", "lowest temp", or "middle temp"), and explain what's happening in that stage (1 sentence per stage).
3) What region(s) of the human genome do the Josh primers that we use in lab amplify?
4) What is the machine called that we run PCRs in? What were the cycling parameters (=actual temps, times, and # of cycles)? (Note that you will not need to know/memorize the cycling parameters for our in class exams - focus on learning what happens at each temp/stage of the cycle).

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2 PCR is based on three simple steps required for any DNA synthesis reaction 1 denaturation of the template into single strands 2 annealing of primers to each original strand for new strand synthesis ... View the full answer