Can we work on this problem

Which of the indicated amino acids within the SH2 domain do you predict forms an electrostatic interaction with the phosphorylated-tyrosine residue in a target protein ? LO 4 ABC D E PE Poll Q4 10 40 50 60 70 .* .. . . ..*. . .. . 446 WEDGVMEVLKKHHKPHWNDGAILGFVNKQQAHDLLINKPDGTELLRFSDSEIGGITIAWKFDSpd-----I p-Tyr 457 WFFSIMOLIKOKLLKFWDEGWCIGFISKNDASQSMMMCqHSSFLLRFSDSQTGAVSIGFVCEEadg---qk 457 WFFSIMOLIKOKLLKYWDEGWCIGFISKHDASQSMCMLPNTSFLLRFSDTQTGAVSIGFVCQDddg---qk 457 WFFSIMOLIKOKLLKFWDEGWCIGFISKNDASQSMMMCqHSSFLLRFSDSQTGAVSIGFVCEEadg---qk 433 WFFKLAEITNKYLYSMWYDGLVYGFCSKEDAENILRCIPRSVLLVRFSDIEYGKIKISVKNRNg---- SH2 625 ELLQIFHDTRNNVRKLWEMGFLMGFMEFEEVDNMLEKH-KSALIMRLSFVTGGTICFTVKSLAhtldprss 529 ELLQIFHDTRNNVRKLWEMGFLMGFMEFEEVDNMLEKH-KSALIMRLSFVTGGTICFTVKSLAhtldprss 276 WFFSIMOLIKOKLLKYWDEGWCIGFISKHDASQSMMMSpHSSFLLRFSDTQTGAVSIGFVCDEegg----k 463 WFFSIMOLIKOKLLKYWDEGWCIGFISKHDASQSMMMSpHSSFLLRFSDTQTGAVSIGFVCDEegg----k 453 WFFEIAALIKOKLLRLWDDGLITGFISREDARSLLLSLhESSFLLRFSDSHLGGRREVFPLAPf- . SH2 domains can bind to phosphorylated-tyrosine residues in various proteins. W = tryptophan, G = glycine, F = phenylalanine, R = arginine D103 Cell Biology ALS 15 @ MacGregor, Suetterlin Wang - All rights reserved Page 5