can you answer these two question?

5. You are binding a fluorescent compound Z-105 to a protein. When monitored at EX=370nm and EM=520nm, a 1uM solution of the compound yields a fluorescence of 20,000 (arbitrary units). When an eqimolar amount of protein is added, the fluorescence at 520 decreases to 15,000 units. If the extinction coefficient and quantum yield of Z-105 are the same when it is bound to the protein as when it is free in solution, explain why the fluorescence changed. a 6. The efficiency of energy transfer (FRET) between a trp residue on a protein and a bound molecule of ANS is 87%. How far apart are the ANS and trp in angstroms