Question: Can you help me solve these two! HINT: HINT: Problem Set 1, question 6 A protein is mixed with ligand. After binding, the remaining concentration

Can you help me solve these two!

HINT: HINT: Problem Set 1, question 6 A protein is mixed with ligand. After binding, the remaining concentration of free ligand is 2 uM. If the stoichiometry of the interaction is 2 ligands/protein, and the K, is 100 nM, then what is the fraction of enzyme that is not bound? (assume that both binding sites are independent) LL] Y = __ This is the fraction of sites occupied (bound). Kd+ [L] Problem Set 1, question 7 Given the Kd for a protein with n equivalent, non-interacting sites, what is the fraction of proteins that have one or more ligands bound as a function of the free ligand concentration upon equilibrium ([L]) ? Use your answer from the previous

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