Question: can you please answer the question as accurately as possible Exercise 1 Enzyme Specificity In this exercise, you will determine the ability of lactase to

can you please answer the question as accurately as possible

can you please answer the question as accurately
Exercise 1 Enzyme Specificity In this exercise, you will determine the ability of lactase to be specific and to catabolize. You will compare the effect of lactase in the breakdown of lactose into glucose and galactose and the breakdown of sucrose into glucose and fructose. Procedure 1 Use the permanent marker to label 4 plastic cups as follows: 5% sucrose, milk, dH0, and lactase. 2 Pour the %cup of milk into the cup labeled "milk', and pour approximately %cup of distilled water into the cup labeled "dH0" As these are approximate amounts itis not necessary to use the graduated cylinder for this step. 3 Use the permanent marker to label the bulb of a clean pipet \"milk\" and place the labeled pipet in the milk cup. Label a second clean pipet "dH,0" and place the labeled pipet into the distilled water cup. 4 Prepare the lactase solution as follows: a Use the graduated cylinder to measure and transfer 150 mL of distilled water into the plastic cup labeled \"lactase: b Crush the lactase pill in its wrapping by placing the pill on a table and using the bottom of the 250 ml glass beaker to crush the tablet. Be gentle with the beaker, taking care not to break it while performing this step. See Figure 6. Figure 6. Crushing the lactase tablet. Carefully open the wrapping of the crushed tablet and pour it into the \"lactase\" cup holding 150 mL of distilled water. d_Use the permanent marker to label the bulb of a clean pipet \"lactase\" and place the pipet into the lactase cup. 5 Use the lactase pipet to stir the crushed lactase pill and distilled water until dissolved. Note: Itis allright if there is additional crushed pill that does not dissolve into the water. 6 Prepare the 5% sucrose solution as follows: a _ Place the weigh boat onto the digital scale and tare the scale so that it reads 0.00 g, b Add sucrose (table sugar) to the weigh boat until scale reads 2.508. Carefully pour the sucrose from the weigh boat into the graduated cylinder. d__ Fillthe graduated cylinder (to the 50.0 mL mark) with distilled water. Pour the contents of the graduated cylinder into the cup labeled \"5% sucrose.\" f Carefully pour the contents of the cup back into the graduated cylinder and then back into the cup, as needed, to transfer all of the sucrose into the labeled cup. 8 _ Use the permanent marker to label a clean pipet \"5% sucrose\" and place the pipet into the cup. h Use tthe pipet to stir the solution until the sucrose is fully dissolved in the distilled water. 7 _ Place all 4 prepared solutions onto your work surface. See Figure 7. Figure 7. Prepared solutions. 8 Place aclean piece of paper towel on your work surface. 9 Use the permanent marker to label 5 wells of the 24-well plate \"A, B, C, D', and \"E." Then place the labeled well plate on top of the paper towel 10 Gather 5 glucose test strips and use the permanent marker to label the strips \"A, B, C, D', and\" Place each glucose stripin front of its corresponding well. 11. Use the scissors to cut off the tip of the 20% glucose pipet, and place the pipet bulb-side down (tip side up) in an empty well of the 24-well plate. The well-plate will act as a pipet holder. 12. Asdescribed in Data Table 1, use the labeled pipets to add solutions to the following wells: a InWell A: add 3 drops of 5% sucrose and 3 drops of dH2O b_ InWell B: add 3 drops of milk and 3 drops of dH, _ InWell C: add 3 drops of 5% sucrose and 3 drops of lactase d__InWell D: add 3 drops of milk and 3 drops of lactase @ InWell E: add 3 drops of 20% glucose and 3 drops of dH>O Note: Ensure that pipets are only used to transfer solutions from their designated cups. 13. Allow each of the reactions to occur for 5 minutes. 14 After 5 minutes, pick up the glucose strip labeled remain in the solution for 5 seconds. nd insert the green end into the reaction in Well A. Allow the st kip to main content emove the strip from the well and place it on the paper towel. 16 Use the to determine the amount of glucose (mmol/L) present in the well. Read the test strip 1 minute after removing it from the well. 17. Use the Glucose Color Chart to determine the amount of glucose (mmol/L) present in the well. Read the test strip 1 minute after removing it from the well, 18 Record the amount of glucose present in Data Table 1 under \"Glucose Concentration\" 19 Repeat steps 13 through 16 for the remaining 4 wells (B,C, D, and E). Cleanup: + Discard the used glucose strips in the trash when complete. '+ Keep all solutions and other materials to use in the next exercises. Exercise 1 - Questions Question 1. Explain why a glucose test strip may be used to determine the enzymatic activity of lactase. BoruetEeEer tT 0 Word(s) Question 2 In this exercise, ive reactions were performed. Of those reactions, two were negative controls and one was a positive control. Describe which of the reactions were controls and if they provided the expected results. Use the data in Data Table 1 to support your answer. Boru s iF 0 Word(s) Question 3 Did the lactase enzyme show specificity? Support your answer by comparing your data for lactose and sucrose. 5 eect 0 Word(s)

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