Chemistry

How to calculate ELISA results? based on the standards are 1000, 500, 250, 125, 62.5, 31.3, 15.6 ,7.8pg/ML,

Transcribed Image Text:

Row data A B с D E F G H Blank subtracted Standard 1 = Standard 2 5 Standard 3 5 Standard 4 Standard 5 3 Standard 6 Standard 7 Standard 8 3 Mean OD Standard 1 5 Standard 2 5 Standard 3 Standard 4 Standard 5 Standard 6 Standard 7 Standard 8 Standard 2.32 1.641 1.0875 0.561 0.3425 0.2425 0.1385 0.108 2.293 1.688 1.132 0.69 0.463 0.348 0.238 0.227 1 2.185 1.579 1.023 0.581 0.355 0.24 0.13 0.119 Standard 1 Standard 2 Standard 3 Standard 4 Standard 5 Standard 6 Standard 7 Standard 8 Sample 1 Sample 2 Sample 3 Sample 4 Sample 5 Sample 6 Sample 7 Sample 8 2.32 Sample 1 1.641 Sample 2 1.0875 Sample 3 0.561 Sample 4 0.3425 Sample 5 0.2425 Sample 6 0.1385 Sample 7 0.108 Sample 8 Concentration 1000 pg/ml 500 pg/ml 250 pg/ml 125 pg/ml 62.5 pg/ml 31.3 pg/ml 15.6 pg/ml 7.8 pg/ml 2.563 1.812 1.26 0.65 0.438 0.354 0.255 0.205 2 2.455 1.703 1.152 0.541 0.33 0.245 0.147 0.097 0.224 0.068 0.415 0.102 0.218 0.133 0.216 0.312 3 0.115 -0.04 0.307 -0.006 0.109 0.024 0.107 0.203 Sample 1 Sample 2 Sample 3 Sample 4 Sample 5 Sample 6 Sample 7 Sample 8 0.218 0.077 0.439 0.104 0.222 0.139 0.208 0.296 4 0.109 -0.031 0.33 -0.005 0.114 0.03 0.1 0.187 Sample 9 Sample 10 Sample 11 Sample 12 bsorbance (450nm) Sample 13 Sample 14 Sample 15 Sample 16 0.112 Sample 9 -0.0355 Sample 10 0.3185 Sample 11 -0.0055 Sample 12 0.1115 Sample 13 0.027 Sample 14 0.1035 Sample 15 0.195 Sample 16 2.5- 2.0- 1.5- 1.0- 0.5- 0.066 0.17 0.176 0.843 2.353 0.104 0.062 1.591 5 -0.042 0.061 0.068 0.735 2.245 -0.005 -0.046 1.482 Sample 9 Sample 10 Sample 11 Sample 12 Sample 13 Sample 14 Sample 15 Sample 16 0.048 0.17 0.189 0.909 2.472 0.114 0.07 1.657 6 -0.06 0.061 0.081 0.801 2.364 0.006 -0.038 1.548 Sample 21 Sample 22 Sample 23 Sample 24 0.157 0.065 -0.051 Sample 17 0.061 Sample 18 0.0745 Sample 19 0.768 Sample 20 2.3045 Sample 21 0.0005 Sample 22 -0.042 Sample 23 1.515 Sample 24 | 0.072 0.424 Data 1 0.091 0.116 Sample 17 Sample 17 Sample 18 Sample 18 Sample 19 Sample 19 Sample 20 Sample 20 0.248 0.049 7 0.049 -0.043 -0.036 0.315 -0.018 0.007 0.139 -0.06 Sample 21 Sample 22 Sample 23 Sample 24 0.14 0.062 0.074 0.436 0.087 0.121 0.238 0.049 8 0.031 -0.047 -0.034 0.328 -0.021 0.013 0.13 -0.059 Sample 25 Sample 26 Sample 27 Sample 28 Sample 29 Sample 30 Sample 31 Sample 32 0.04 Sample 25 -0.045 Sample 26 -0.035 Sample 27 0.3215 Sample 28 -0.0195 Sample 29 0.01 Sample 30 0.1345 Sample 31 -0.0595 Sample 32 0.168 0.062 0.064 0.296 0.252 0.099 0.078 0.136 9 0.059 -0.047 -0.045 0.187 0.144 -0.01 -0.03 0.027 0.167 0.06 0.063 0.298 0.338 0.092 0.073 0.146 10 0.058 -0.049 -0.045 0.19 0.229 -0.016 -0.035 0.038 Sample 25 Sample 33 Sample 26 Sample 34 Sample 27 Sample 35 Sample 28 Sample 36 Sample 29 Blank Sample 30 Blank Sample 31 Blank Sample 32 Blank 0.0585 Sample 33 -0.048 Sample 34 -0.045 Sample 35 0.1885 Sample 36 0.1865 Blank -0.013 Blank -0.0325 Blank 0.0325 Blank 0.175 0.183 0.111 0.111 0.079 0.079 0.17 0.184 0.096 0.098 0.098 0.106 0.15 0.128 0.097 0.094 11 0.067 0.003 -0.029 0.062 -0.012 -0.01 -0.011 -0.015 Sample 33 Sample 34 Sample 35 Sample 36 Blank Blank Blank Blank 0.0705 0.003 -0.029 0.0685 -0.011 -0.0065 0.015 0.0025 450 450 450 450 450 450 450 12 0.074 Blank 450 0.003 Blank 450 -0.029 Blank 450 0.075 Blank 450 -0.01 Blank 450 -0.003 Blank 450 0.041 Blank 450 0.02 Blank 450 Row data A B с D E F G H Blank subtracted Standard 1 = Standard 2 5 Standard 3 5 Standard 4 Standard 5 3 Standard 6 Standard 7 Standard 8 3 Mean OD Standard 1 5 Standard 2 5 Standard 3 Standard 4 Standard 5 Standard 6 Standard 7 Standard 8 Standard 2.32 1.641 1.0875 0.561 0.3425 0.2425 0.1385 0.108 2.293 1.688 1.132 0.69 0.463 0.348 0.238 0.227 1 2.185 1.579 1.023 0.581 0.355 0.24 0.13 0.119 Standard 1 Standard 2 Standard 3 Standard 4 Standard 5 Standard 6 Standard 7 Standard 8 Sample 1 Sample 2 Sample 3 Sample 4 Sample 5 Sample 6 Sample 7 Sample 8 2.32 Sample 1 1.641 Sample 2 1.0875 Sample 3 0.561 Sample 4 0.3425 Sample 5 0.2425 Sample 6 0.1385 Sample 7 0.108 Sample 8 Concentration 1000 pg/ml 500 pg/ml 250 pg/ml 125 pg/ml 62.5 pg/ml 31.3 pg/ml 15.6 pg/ml 7.8 pg/ml 2.563 1.812 1.26 0.65 0.438 0.354 0.255 0.205 2 2.455 1.703 1.152 0.541 0.33 0.245 0.147 0.097 0.224 0.068 0.415 0.102 0.218 0.133 0.216 0.312 3 0.115 -0.04 0.307 -0.006 0.109 0.024 0.107 0.203 Sample 1 Sample 2 Sample 3 Sample 4 Sample 5 Sample 6 Sample 7 Sample 8 0.218 0.077 0.439 0.104 0.222 0.139 0.208 0.296 4 0.109 -0.031 0.33 -0.005 0.114 0.03 0.1 0.187 Sample 9 Sample 10 Sample 11 Sample 12 bsorbance (450nm) Sample 13 Sample 14 Sample 15 Sample 16 0.112 Sample 9 -0.0355 Sample 10 0.3185 Sample 11 -0.0055 Sample 12 0.1115 Sample 13 0.027 Sample 14 0.1035 Sample 15 0.195 Sample 16 2.5- 2.0- 1.5- 1.0- 0.5- 0.066 0.17 0.176 0.843 2.353 0.104 0.062 1.591 5 -0.042 0.061 0.068 0.735 2.245 -0.005 -0.046 1.482 Sample 9 Sample 10 Sample 11 Sample 12 Sample 13 Sample 14 Sample 15 Sample 16 0.048 0.17 0.189 0.909 2.472 0.114 0.07 1.657 6 -0.06 0.061 0.081 0.801 2.364 0.006 -0.038 1.548 Sample 21 Sample 22 Sample 23 Sample 24 0.157 0.065 -0.051 Sample 17 0.061 Sample 18 0.0745 Sample 19 0.768 Sample 20 2.3045 Sample 21 0.0005 Sample 22 -0.042 Sample 23 1.515 Sample 24 | 0.072 0.424 Data 1 0.091 0.116 Sample 17 Sample 17 Sample 18 Sample 18 Sample 19 Sample 19 Sample 20 Sample 20 0.248 0.049 7 0.049 -0.043 -0.036 0.315 -0.018 0.007 0.139 -0.06 Sample 21 Sample 22 Sample 23 Sample 24 0.14 0.062 0.074 0.436 0.087 0.121 0.238 0.049 8 0.031 -0.047 -0.034 0.328 -0.021 0.013 0.13 -0.059 Sample 25 Sample 26 Sample 27 Sample 28 Sample 29 Sample 30 Sample 31 Sample 32 0.04 Sample 25 -0.045 Sample 26 -0.035 Sample 27 0.3215 Sample 28 -0.0195 Sample 29 0.01 Sample 30 0.1345 Sample 31 -0.0595 Sample 32 0.168 0.062 0.064 0.296 0.252 0.099 0.078 0.136 9 0.059 -0.047 -0.045 0.187 0.144 -0.01 -0.03 0.027 0.167 0.06 0.063 0.298 0.338 0.092 0.073 0.146 10 0.058 -0.049 -0.045 0.19 0.229 -0.016 -0.035 0.038 Sample 25 Sample 33 Sample 26 Sample 34 Sample 27 Sample 35 Sample 28 Sample 36 Sample 29 Blank Sample 30 Blank Sample 31 Blank Sample 32 Blank 0.0585 Sample 33 -0.048 Sample 34 -0.045 Sample 35 0.1885 Sample 36 0.1865 Blank -0.013 Blank -0.0325 Blank 0.0325 Blank 0.175 0.183 0.111 0.111 0.079 0.079 0.17 0.184 0.096 0.098 0.098 0.106 0.15 0.128 0.097 0.094 11 0.067 0.003 -0.029 0.062 -0.012 -0.01 -0.011 -0.015 Sample 33 Sample 34 Sample 35 Sample 36 Blank Blank Blank Blank 0.0705 0.003 -0.029 0.0685 -0.011 -0.0065 0.015 0.0025 450 450 450 450 450 450 450 12 0.074 Blank 450 0.003 Blank 450 -0.029 Blank 450 0.075 Blank 450 -0.01 Blank 450 -0.003 Blank 450 0.041 Blank 450 0.02 Blank 450

Answer rating: 100% (QA)

We calculate ELISA S I S ELISA j... View the full answer