Consider a culture of bacteria with empirical biomass formula CH1.700.46 No.18 (MWB = 23.6 g/mole) growing...

 

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Consider a culture of bacteria with empirical biomass formula CH1.700.46 No.18 (MWB = 23.6 g/mole) growing aerobically on glucose (C6H₁2O6). You decide to measure the yield coefficients for glucose and oxygen and find that Yx/s 85 g biomass/mole glucose; Yx/02= 40 g biomass/mole O₂. This organism secretes no appreciable amounts of product under these conditions. a) (15 pts) Show that the measured values of Yxs and Yx/o2 are stoichiometrically consistent with each other, stating all assumptions.co b) (15 pts) A batch culture of this organism initially contains 0.01 g of biomass inoculum and 20 mmol glucose. The culture is incubated overnight, and subsequent optical density measurements suggest that the cells are no longer growing. The total biomass in the culture is calculated to be 1.0 g. Estimate the final amount of glucose in the medium (mmol), and speculate on what caused the plateau in biomass amount. Consider a culture of bacteria with empirical biomass formula CH1.700.46 No.18 (MWB = 23.6 g/mole) growing aerobically on glucose (C6H₁2O6). You decide to measure the yield coefficients for glucose and oxygen and find that Yx/s 85 g biomass/mole glucose; Yx/02= 40 g biomass/mole O₂. This organism secretes no appreciable amounts of product under these conditions. a) (15 pts) Show that the measured values of Yxs and Yx/o2 are stoichiometrically consistent with each other, stating all assumptions.co b) (15 pts) A batch culture of this organism initially contains 0.01 g of biomass inoculum and 20 mmol glucose. The culture is incubated overnight, and subsequent optical density measurements suggest that the cells are no longer growing. The total biomass in the culture is calculated to be 1.0 g. Estimate the final amount of glucose in the medium (mmol), and speculate on what caused the plateau in biomass amount.

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Glucose C6H06 MW cell mass CH170046 No 18 MW biomass ... View the full answer