Question: Consider a yeast cell which wants to express a 900 bp gene. Assuming this gene has no introns, ignoring any 5 or 3 untranslated regions,

Consider a yeast cell which wants to express a 900 bp gene. Assuming this gene has no introns, ignoring any 5’ or 3’ untranslated regions, and assuming a 100 bp poly-A tail, how many nucleotides are required to transcribe this gene? For simplicity’s sake, let’s assume all the nucleotides are ATP.

Based on what you know about translation, how many ATPs (or GTPs) would required to translate this protein?

Given the total number of ATPs required for transcription and translation, how many glucose molecules would be required to generate enough ATPs?

If the total amount of protein in the yeast cell is 10-20 mol, how much glucose is required to synthesize all the cellular protein (assume all proteins are identical to the one you’ve calculated in steps 1-3)?

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