Consider the following complementation test strains and their results. The unknown his mutants have single mutations in different his genes.

Tested strains:

Strain 1: his 5 Strain A: his #?

Strain 2: his 1 Strain B: his #?

Strain 3: his 7 Strain C: his #?

Results: Media: SD + histidine

Strain 1 X A: growth Strain 2 X A: no growth Strain 3 X A: no growth

Strain 1 X B: growth Strain 2 X B: growth Strain 3 X B: growth

Strain 1 X C: growth Strain 2 X C: no growth Strain 3 X C: no growth

Results: Media: SD

Strain 1 X A: growth Strain 2 X A: no growth Strain 3 X A: no growth

Strain 1 X B: no growth Strain 2 X B: growth Strain 3 X B: growth

Strain 1 X C: growth Strain 2 X C: no growth Strain 3 X C: no growth

What can you conclude from the test results? Explain your reasoning in detail.

Next to each mating, write the known diploid genotype. Assume the same TRP/trp and ADE/ade mutations for all strains just like the strains used in the experiment (from the lab manual).