Consider the scale up of a chromatography column for purification of protein. A column of 40...
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Consider the scale up of a chromatography column for purification of protein. A column of 40 cm length is used with a superficial velocity of 40 cm/hr. The peak concentration of the target protein is at a time of 100 min. the standard deviation of the distribution is 14 min. a. If you collect your chromatography elution at t1=0, How long must you wait to collect 90% yield of the protein? b. If the same column is used, but velocity is increased to 60 cm/hr and internal diffusion controlled, what will be the value of the new ? c. If the same column is lengthened to 60cm while increase the velocity is at 60 cm/hr, how will o change compare to the original condition (40 cm/h with a 40- cm column)? Consider the scale up of a chromatography column for purification of protein. A column of 40 cm length is used with a superficial velocity of 40 cm/hr. The peak concentration of the target protein is at a time of 100 min. the standard deviation of the distribution is 14 min. a. If you collect your chromatography elution at t1=0, How long must you wait to collect 90% yield of the protein? b. If the same column is used, but velocity is increased to 60 cm/hr and internal diffusion controlled, what will be the value of the new ? c. If the same column is lengthened to 60cm while increase the velocity is at 60 cm/hr, how will o change compare to the original condition (40 cm/h with a 40- cm column)?
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