Describe restriction endonucleases? Give examples of commonly used restriction endonucleases. What is restriction site? Define transformation. Define
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- Describe restriction endonucleases? Give examples of commonly used restriction endonucleases.
- What is restriction site?
- Define transformation.
- Define plasmids. Give examples
- Describe molecula cloning. Step by step. You can illustrate with diagrams.
- Give 5 examples of sources of genetic material such as blood for testing. Make comments.
- What are the differences between NGS and Sanger sequencing.
- Describe methods for scanning a gene for mutations before sequencing. Write down advantages and disadvantages.
- Unclassified variants are a problem for genetic testing. Please list the suggestions fort his problem to be solved.
- Summarise 3 methods for testing for a specific mutation or SNP.
- Describe recombinant DNA technology?
- What are the main goals of recombinant DNA technology?
- List the tools in recombinant DNA technology.
- Describe CLONING methodology.
- What are cloning vectors. Why plasmids are good cloning vectors.
- What are the disadvantages using plasmids?
- What are the applications of recombinant DNA technology?
- Define ethics and safety in recombinant DNA technology. Describe ethical issues.
- Describe PCR DNA Sequencing.
- Describe FISH.
- Describe Microarray technology
- What is genome editing?
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