Detection of alpha synuclein $$on $$AuIDEs without surface modification and analysis of linear regression based on current$-$voltage measurement.For each concentration interacted, we took current$-$voltage measurements, recording values of $1\mathrm{.}0$ x $10-5,1\mathrm{.}5$ x $10-5,3\mathrm{.}7$ x $10-5,4\mathrm{.}2$ x $10-5,4\mathrm{.}6$ x $10-5,4\mathrm{.}7$ x $10-5,$ and $4\mathrm{.}9$ x $10-5$ A$.$ A linear regression yielded an R$2$ value of $0\mathrm{.}7796($Figure $4$a$).$ I$-$V analyzer measurements ranging from aM to pM were used to immobilize and identify the aggregations. The concentrations of interest were diluted to $1$ aM$,10$ aM$,100$ aM$,1$ fM$,10$ fM$,100$ fM$,$ and $1$ pM$.$ Following the completion of the dilution, each concentration was immobilized. Figure $4$b depicts the current flow values corresponding to aggregated alpha$-$synuclein interaction on the surface of the AuIDEs, which were recorded as $2\mathrm{.}6$ x $10-5,2\mathrm{.}8$ x $10-5,3\mathrm{.}6$ x $10-5,3\mathrm{.}8$ x $10-5,4\mathrm{.}4$ x $10-5,4\mathrm{.}6$ x $10-5,$ and $5$ x $10-5,$ respectively; a linear regression analysis yielded an R$2$ value of $0\mathrm{.}9779$