Question: Determine Nucleotide and protein changes from red and white ADE2 multiple sequence alignments. Identify the type of mutation present in the coding sequence and discuss

CLUSTAL O(1.2.4) multiple sequence alignment NhitecodingADE2 RedcodingADE2 MDSRIVGILGOGQLGRMIVEAANRINIKTVILDAENSPAKQISNSNDHVNGSFSNPLDIE 60 MDSRTVGILGOGOLGRMIVEAANRINIKTVILDAENSPAKQISNSNDHVNGSESNPLDIE 60 ****** Determine Nucleotide and protein changes from red and white ADE2 multiple sequence alignments.

Identify the type of mutation present in the coding sequence and discuss the implications of the protein.

White coding sequence: White Yeast

Red coding sequence: Red yeast

ADE2: gene

Dash marks in the sequence represent deletion or mutation.

Pictures 2 and 3 (DNA alignments) numbered 60-1716

Picture 1 (Protein alignments) numbered 60-570

120 NhitecodingADE2 RedcodingADE2 KLAEKCDVLTIEIEHVDVPTLKNLOVKHPKLKIYPSPETIRLIQOKYIQKEHLIKNGIAV KLA-XCDVLTIEIEHVDVPTLKNLOVKHPKLKIYPSPETIRLIQDKYIQKEHLIKNGIAV 119 ******** ***** NhitecodingADE2 RedcodingADE2 TOSVPVEQASETSLINVGRDLGFPEVLKSRILAYDGRGNEVVENKEMIPEALEVLXDRPL TOSVPVEQASETSLINVGRDLGFPEVLKSRILAYDGRGNEVVKNKEMIPEALEVLXDRPL 180 179 NhitecodingADE2 RedcodingADE2 YAEKWAPFTKELAVMIVRSVNGLVFSYPIVETIHKDNICDLCYAPARVPDSVOLKAKLLA YAEKHAPFTKELAVMIVRSVNGLVFSYPIVETIHKDNICDLCYAPARVPDSVOLKAKLLA 240 239 ..**** STGELLINEIAPRPHNSCHYTIDACVTSQFEAHLRSILDL. ENAIKSFPGOGIFGVEMFYLETGELLINEIAPRPHNSCHYTIDACVTSQFEAHLRSILDL 300

CLUSTAL O(1.2.4) multiple sequence alignment NhitecodingADE2 RedcodingADE2 MDSRIVGILGOGQLGRMIVEAANRINIKTVILDAENSPAKQISNSNDHVNGSFSNPLDIE 60 MDSRTVGILGOGOLGRMIVEAANRINIKTVILDAENSPAKQISNSNDHVNGSESNPLDIE 60 ****** 120 NhitecodingADE2 RedcodingADE2 KLAEKCDVLTIEIEHVDVPTLKNLOVKHPKLKIYPSPETIRLIQOKYIQKEHLIKNGIAV KLA-XCDVLTIEIEHVDVPTLKNLOVKHPKLKIYPSPETIRLIQDKYIQKEHLIKNGIAV 119 ******** ***** NhitecodingADE2 RedcodingADE2 TOSVPVEQASETSLINVGRDLGFPEVLKSRILAYDGRGNEVVENKEMIPEALEVLXDRPL TOSVPVEQASETSLINVGRDLGFPEVLKSRILAYDGRGNEVVKNKEMIPEALEVLXDRPL 180 179 NhitecodingADE2 RedcodingADE2 YAEKWAPFTKELAVMIVRSVNGLVFSYPIVETIHKDNICDLCYAPARVPDSVOLKAKLLA YAEKHAPFTKELAVMIVRSVNGLVFSYPIVETIHKDNICDLCYAPARVPDSVOLKAKLLA 240 239 ..**** STGELLINEIAPRPHNSCHYTIDACVTSQFEAHLRSILDL. ENAIKSFPGOGIFGVEMFYLETGELLINEIAPRPHNSCHYTIDACVTSQFEAHLRSILDL 300 WhitecodingADE2 RedcodingADE2 ENAIKSFPGOGIFGVE 299 ****** WhitecodingADE2 RedcodingADE2 PMPKNETSFSTITINA TKDKELETCERALATPGSSVYLYGKESRPNRKVG 360 PMFKNFTSFSTITINAIMLNVLGDKHTKDKELETCERALATPGSSVYLYGKESRPNRKVG 359 ****** NhitecodingADE2 RedcodingADE2 HINIIASSMAECEQRLNYITGRIDIPIKISVAQKLDLEMVKPLVGIIMGSDSDLPVMSA HINIIASSMABCEQRINYITGRIDIPIKISVAGKLDLEAMVKPLVGIIMGSDSDLPVMSA 420 419 ****** WhitecodingADE2 RedcodingADE2 NCAVLKDFGVPFEVTIVSAHRTPHRMSAYAISASKRGIKTIIGAGGAAHLPGMVAAMTP 480 NCAVLEDFGVPFEVTIVSAHRTPHRMSAYAISASKRGIKTIIAGAGGAAHLPGVAAMTP 479 ****** WhitecodingADE2 RedcodingADE2 LPVIGVPVKGSCLDGVDSLESIVOMPRGVEVATVAINNSTNAALLAVRLLGAYDSSYTTK LPVIGVPVKGSCLDGVDSLESIVOMPRGVPVATVAINNSTNAALLAVRLLGAYDSSYTTK 540 539 WhitecodingADE2 RedcodingADE2 MEQFLLKOEEEVLVKAQKLETVGYEAYLENK MEQFLLKQEEEVLVKAQKLETVGYEAYLENK 571 570

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