Determine the optimal incubation condition for HaCat cells to grow? Answer all questions and record all calculations

Determine the optimal incubation condition for HaCat cells to grow?

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Answer all questions and record all calculations in your lab book. 1. Calculate the appropriate amount of reagents necessary to prepare a complete RPMI media using the following recipe. RPMI 1640 medium Starting concentration FBS 100% Non essential amino acids 10X 100U/ml penicillin and 10X streptomycin sulphate Concentration required 10% 1X 1X Complete RPMI (500ml) Answer all questions and record all calculations in your lab book. 1. Calculate the appropriate amount of reagents necessary to prepare a complete RPMI media using the following recipe. RPMI 1640 medium Starting concentration FBS 100% Non essential amino acids 10X 100U/ml penicillin and 10X streptomycin sulphate Concentration required 10% 1X 1X Complete RPMI (500ml)

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Determine the optimal incubation condition for HaCat cells to grow The optimal incubation condition for HaCat cells to grow is 37C with 5 CO2 The optimal incubation condition for HaCat cells to grow i... View the full answer