Determine the Protein Concentration using the linear equation from the Standard Curve from the Biuret Protein Assay.

Tube	Absorbance 1 (540nm)	Absorbance 2 (540nm)	Average	Protein Concentration (mg/mL)
1 (blank)	0.05	0.04	0.045	0
2	0.06	0.06	0.06	0.4
3	0.1	0.08	0.04	0.8
4	0.11	0.1	0.105	1.2
5	0.15	0.13	0.14	1.6
6	0.16	0.15	0.155	2
Unknown Protein 1	0.09	0.09	0.09
Unknown Protein 2	0.09	0.09	0.09
Unknown Protein 3	0.07	0.07	0.07

Standard Curve: Biuret Assay 0.18 0.16 0.14 0.12 0.1 Protein Concentration (mg/mL) 0.08 0.06 0.04 y = 0.0611x + 0.0298 R2 = 0.8442 0.02 0 0 0.5 1 1.5 2 2.5 Absorbance (540nm)