do all 3 please

Hemacytometer Pre-Lab (Use Counting Cells in the Plating Cells Protocol for reference) 1. Box the four squares on the grid that you are going to count. 2. Count at least 3 out of the 4 boxes on one side of the hemacytometer and get the average cells/mL. (the average of your counts 104 gives the cell concentration per mL. a. Example: Hema count: 133,145,128,154 Avg =140,140104 cells /mL b. When you are counting your cells, you will also count the boxes on the opposite side of the hemacytometer. Hema count: Avg = Cells /mL= 3. Find the number of mL you need from your flask of cells to get the total number of cells needed. a. Example: averagecells/mLtotalcellsneeded1.4106cells/mL8106cells=5.7mL of cells Talee this amount frow flask of cells and add to a centrifuge tube - You are plating 20 wells with a concentration of 2105 cells/well. You will put 2mL of cell suspension (cells from flask + media) in each well. (Don't forget to make extra.) Total number of cells needed: Total volume: Volume needed from the flask of cells: Volume of media needed to add to cells