Question: Do the calculations to make the required buffer 25 mM Tris-HCl, pH 8.3; 192 mM glycine, 0.1% SDS buffer in 250 mL. Show detailed work

Do the calculations to make the required buffer 25 mM Tris-HCl, pH 8.3; 192 mM glycine, 0.1% SDS buffer in 250 mL. Show detailed work and describe step-by-step procedures for making this buffer.

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