Question: Do your time-course plots begin linearly, then level off as the reactions proceed? 2. Do your Michaelis-Menten and Lineweaver-Burk plots resemble the classical versions of
Do your time-course plots begin linearly, then level off as the reactions proceed? 2. Do your Michaelis-Menten and Lineweaver-Burk plots resemble the classical versions of these plots? If not, explain what could have caused any deviations from the expected results. 3. Hopefully, the Km and Vmax values from the Michaelis-Menten and Lineweaver-Burk plots will be in close agreement. If this is not the case, explain what could have caused the discrepancy. 4. Why do you suppose Km values are so frequently standardized and published, drawing attention to the value Vmax/2, rather than Vmax itself
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