2. below. (a) (i) In the determination of acetone in biological fluids the following calibration curve...
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2. below. (a) (i) In the determination of acetone in biological fluids the following calibration curve and unknown data obtained are given in the table Standard Mg acetone 100ml Reagent blank 2.0 4.0 6.0 8.0 10.0 Normal blood Ketonic blood Normal urine Ketonic urine (diluted) Absorbance 0.053 0.097 0.143 0.190 0.221 0.238 0.119 0.222 0.150 0.230 All the absorbance measurements were against a water blank. Determine the ketone concentration in each of the four unknowns and assuming a 1:25 (initial: final) dilution was used. Calculate the ketone concentration in the undiluted urine. (7 marks) 2. below. (a) (i) In the determination of acetone in biological fluids the following calibration curve and unknown data obtained are given in the table Standard Mg acetone 100ml Reagent blank 2.0 4.0 6.0 8.0 10.0 Normal blood Ketonic blood Normal urine Ketonic urine (diluted) Absorbance 0.053 0.097 0.143 0.190 0.221 0.238 0.119 0.222 0.150 0.230 All the absorbance measurements were against a water blank. Determine the ketone concentration in each of the four unknowns and assuming a 1:25 (initial: final) dilution was used. Calculate the ketone concentration in the undiluted urine. (7 marks)
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