Exercise #1 The following enzymatic kinetic data are provided below. Kinetic data were collected without using inhibitor and later with the use of an inhibitor at two different doses as indicated in the table below. 1) Plot the rate of reactions as function of the substrate concentration (without and with inhibitor). Interpret the plot and indicate if the data can indicate the type of inhibitor used 2) Assume that the kinetic rate follows Michaelis Menten type, obtain the linearized form and determine the kinetic parameters (Vmax and Km) 3) Using the linearized plots for all three cases, can you identify the type of inhibitor? 4) Determine the corresponding Ki. Inhibitor nM 0 25 50 V micro V micro S micro M V micro M/min M/min M/ min 0.4 0.22 0.21 02 0.67 0 29 026 0.24 0.32 1 0.3 0.28 04 0.36 0 32 2