Fifty microliters of a 1 100 dilution of a sample were added to 3 ml of Bradford reagent The absorbance at 595 nm was 0 328, which corresponds to 20 0 g protein according to a standard curve What is the protein concentration of the original stock solution in mg ml ...

Answer Given that the absorbance corresponds to 20 g of prot ...

Fifty microliters of a 1:100 dilution of a sample were added to 3 ml of Bradford reagent.

Fantastic news! We've Found the answer you've been seeking!

Question:

Fifty microliters of a 1:100 dilution of a sample were added to 3 ml of Bradford reagent. The absorbance at 595 nm was 0.328, which corresponds to 20.0 μg protein according to a standard curve.

What is the protein concentration of the original stock solution in mg/ml ?

Related Book For answer-question

Statistics for the Life Sciences

ISBN: 978-0321989581

5th edition

Authors: Myra Samuels, Jeffrey Witmer, Andrew Schaffner

See More Books

Posted Date: Feb 16, 2020 11:51 PM

See More Questions

In Experiment 1, 200.0 mL of solution containing 10.0 mg of SO42- (from Na2SO4) were treated with excess BaCl2 solution to precipitate BaSO4 containing some coprecipitated Cl_. To find out how much...
A 200 mg sample of radioactive polonium-2 1 0 is observed as it decays. Table 7.9 shows the mass remaining at various times. Assuming an exponential decay model, use least squares to find the...
To calibrate a standard curve for assaying protein concentrations, a plant pathologist used a spectrophotometer to measure the absorbance of light (wavelength 500 nm) by a protein solution. The...
Chunky Cheese Pizza has $60 million in bonds payable. The bond indenture states that the debt to equity ratio cannot exceed 3.0. Chunky's total assets are $200 million, and its liabilities other than...
In what situations may a gain due to an involuntary conversion of real property be deferred if like-kind property is purchased to replace the converted property?
Ralph Lauren Corporation (RL) designs, markets, and distributes a variety of apparel, home dÃ©cor, accessory, and fragrance products. The companys products include such brands as Ralph...
SWIGART v. BRUNO CALIFORNIA COURT OF APPEALS 13 CAL. APP. 5TH 529 2017 According to the American Endurance Ride Conference, endurance riding is a highly competitive and demanding sport. It is...
The Darby Company manufactures and distributes meters used to measure electric power consumption. The company started with a small production plant in El Paso and gradually built a customer base...
1-2. Draw graphs of the following functions using transformations: 1. y = 2x-1 x-2' 2. y log2x+1|- 3.
Chumpy Lighting Limited manufactures a wide variety of light bulbs which it sells to lighting shops and builders merchants through wholesale distributors. It also sells direct to the big UK...
The most effective approaches to impression management include which of these? Select all that apply. Question 18 options: maintain authenticity describe yourself accurately build credibility be true...
Principal = $1,000; Coupon interest rate = 10%, paid valuing it = 12%. Issued at 92.8 (i.e., 92.8% of face value). annually; Maturity = 5 years. At issuance, cash increases by $928 and bonds payable...
If fixed cost rate is $19,180. clocks sell for $22.99. the breakeven point is$7,000, what is the time's unit cost?
Stocks of the Binomial Inc. are currently $50 per share and it is known that its stock price will either increase by 15% per year or decrease by 25% per year, independently for the next two years....
16. The radiator of a car that is at 110C transfers energy into the 30.0C envi- ronment. Calculate the rate of heat transfer by radiation if the radiator has an emissivity of 0.750 and a surface area...
An investment offers to quadruple your money in 18 months (don't believe it) What rate per three months are you being offered? (Do not round intermediate calculations and enter your answer as a...
Compare the theories of Natural Law and Positivism, using examples, such as legal cases. Describe the two theories and discuss cases which follow one or the other. You can mention the theorists for...
Respond to the ethical judgments required based on the following scenarios. Scenario 1. Assume you have collected a sample using MUS and that you have evaluated that sample to calculate a total...
Compute the standard error of (1 - 2) for the following data: Sample 1 10 125 44.2 Sample 2 10 217 rt 28.7
A sample of 20 fruitfly (Drosophila melanogaster) larva were incubated at 37°C for 30 minutes. It is theorized that such exposure to heat causes polytene chromosomes located in the salivary...
In a study of the tufted titmouse (Parus bicolor), an ecologist captured seven male birds, measured their wing lengths and other characteristics, and then marked and released them. During the ensuing...
Explain the steps in forming a limited liability corporation.
Explain the role played by a board of directors of a corporation.
Define a closely held corporation.

Fifty microliters of a 1:100 dilution of a sample were added to 3 ml of Bradford reagent.

Question:

Expert Answer:

Answer Given that the absorbance corresponds to 20 g of prot... View the full answer

Statistics for the Life Sciences

Students also viewed these chemistry questions