Question: find Vmax Question 1 [20] A research group discovers a new enzyme, happyase that catalyses the chemical reaction HAPPY = SAD The researchers begin to

find Vmax Question 1 [20] A research group discovers a new

find Vmax

Question 1 [20] A research group discovers a new enzyme, happyase that catalyses the chemical reaction HAPPY = SAD The researchers begin to characterize the enzyme. 1.1 In the first experiment, with [E]t at 5.5 nM they find that the Vmax is 6.3 M.s. Based on this experiment what is the kcat for happyase? (Include appropriate units.) (5) 1.2 In another experiment, with [E]: at 1.75 nM, and [HAPPY] at 27.5 um, the researchers find that Vo = 275 nM.s. What is the measured Km of happyase for its substrate HAPPY? (Include appropriate units.)

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